The FREQ Procedure

The data set Pain contains hypothetical data for a clinical trial of a drug therapy to control pain. The clinical trial investigates whether adverse responses increase with larger drug doses. Subjects receive either a placebo or one of four drug doses. An adverse response is recorded as Adverse='Yes'; otherwise, it is recorded as Adverse='No'. The number of subjects for each drug dose and response combination is contained in the variable Count.

data pain;
   input Dose Adverse $ Count @@;
   datalines;
0 No 26   0 Yes  6
1 No 26   1 Yes  7
2 No 23   2 Yes  9
3 No 18   3 Yes 14
4 No  9   4 Yes 23
;

The following PROC FREQ statements provide a trend analysis. The TABLES statement requests a table of Adverse by Dose. The MEASURES option produces measures of association, and the CL option produces confidence limits for these measures. The TREND option tests for a trend across the ordinal values of the variable Dose with the Cochran-Armitage test. The PLOTS= option requests a mosaic plot of Adverse by Dose.

The EXACT statement produces exact p-values for this test, and the MAXTIME= option terminates the exact computations if they do not complete within 60 seconds. The TEST statement computes an asymptotic test for Somers’ .

ods graphics on;
proc freq data=Pain;
   tables Adverse*Dose / trend measures cl
          plots=mosaicplot;
   test smdrc;
   exact trend / maxtime=60;
   weight Count;
   title 'Clinical Trial for Treatment of Pain';
run;
ods graphics off;

Output 40.8.1 through Output 40.8.4 display the results of the analysis. The "Col Pct" values in Output 40.8.1 show the expected increasing trend in the proportion of adverse effects with the increasing dosage (from 18.75% to 71.88%). The corresponding mosaic plot (Output 40.8.2) also shows this increasing trend.

Output 40.8.2: Mosaic Plot

Output 40.8.3 displays the measures of association produced by the MEASURES option. Somers’ measures the association treating the row variable (Adverse) as the response and the column variable (Dose) as a predictor. Because the asymptotic 95% confidence limits do not contain zero, this indicates a strong positive association. Similarly, the Pearson and Spearman correlation coefficients show evidence of a strong positive association, as hypothesized.

The Cochran-Armitage test (Output 40.8.4) supports the trend hypothesis. The small left-sided p-values for the Cochran-Armitage test indicate that the probability of the Row 1 level (Adverse='No') decreases as Dose increases or, equivalently, that the probability of the Row 2 level (Adverse='Yes') increases as Dose increases. The two-sided p-value tests against either an increasing or decreasing alternative. This is an appropriate hypothesis when you want to determine whether the drug has progressive effects on the probability of adverse effects but the direction is unknown.