This example illustrates how you can construct a bootstrap confidence interval by using the multiple responses feature in PROC TPSPLINE.
Numerous epidemiological observations have indicated that exposure to solar radiation is an important factor in the etiology of melanoma. The following data present ageadjusted melanoma incidences for 37 years from the Connecticut Tumor Registry (Houghton, Flannery, and Viola, 1980). The data are analyzed by Ramsay and Silverman (1997).
data melanoma; input year incidences @@; datalines; 1936 0.9 1937 0.8 1938 0.8 1939 1.3 1940 1.4 1941 1.2 1942 1.7 1943 1.8 1944 1.6 1945 1.5 1946 1.5 1947 2.0 1948 2.5 1949 2.7 1950 2.9 1951 2.5 1952 3.1 1953 2.4 1954 2.2 1955 2.9 1956 2.5 1957 2.6 1958 3.2 1959 3.8 1960 4.2 1961 3.9 1962 3.7 1963 3.3 1964 3.7 1965 3.9 1966 4.1 1967 3.8 1968 4.7 1969 4.4 1970 4.8 1971 4.8 1972 4.8 ;
The variable incidences
records the number of melanoma cases per people for the years 1936 to 1972. The following model fits the data and requests a 90% Bayesian confidence interval along
with the estimate:
ods graphics on; proc tpspline data=melanoma plots(only)=(criterionplot fitplot(clm)); model incidences = (year) /alpha = 0.1; output out = result pred uclm lclm; run;
The output is displayed in Output 96.5.1
Output 96.5.1: Output from PROC TPSPLINE for the MELANOMA Data
Comparison of Two Estimates 
with and without the D= Option 
Summary of Input Data Set  

Number of NonMissing Observations  37 
Number of Missing Observations  0 
Unique Smoothing Design Points  37 
Summary of Final Model  

Number of Regression Variables  0 
Number of Smoothing Variables  1 
Order of Derivative in the Penalty  2 
Dimension of Polynomial Space  2 
Summary Statistics of Final Estimation  

log10(n*Lambda)  0.0607 
Smoothing Penalty  0.5171 
Residual SS  1.2243 
Tr(IA)  22.5852 
Model DF  14.4148 
Standard Deviation  0.2328 
GCV  0.0888 
The estimated curve is displayed with 90% confidence interval bands in Output 96.5.2. The number of melanoma incidences exhibits a periodic pattern and increases over the years. The periodic pattern is related to sunspot activity and the accompanying fluctuations in solar radiation.
Output 96.5.2: PROC TPSPLINE Estimate and 90% Confidence Interval of Data Set MELANOMA
Wang and Wahba (1995) compare several bootstrap confidence intervals to Bayesian confidence intervals for smoothing splines. Both bootstrap and Bayesian confidence intervals are acrossthecurve intervals, not pointwise intervals. They concluded that bootstrap confidence intervals work as well as Bayesian intervals concerning average coverage probability. Additionally, bootstrap confidence intervals appear to be better for small sample sizes. Based on their simulation, the “percentilet interval” bootstrap interval performs better than the other types of bootstrap intervals.
Suppose that and are the estimates of f and from the data. Assume that is the “true” f, and generate the bootstrap sample as

where . Denote as the random variable of the bootstrap estimate at . Repeat this process K times, so that at each point , you have K bootstrap estimates or K realizations of . For each fixed , consider the statistic , which is similar to the Student’s t statistic,

where is the estimate of based on the ith bootstrap sample.
Suppose and are the lower and upper points, respectively, of the empirical distribution of . The 100% bootstrap confidence interval is defined as

Bootstrap confidence intervals are easy to interpret and can be used with any distribution. However, because they require K model fits, their construction is computationally intensive.
The feature of multiple dependent variables in PROC TPSPLINE enables you to fit multiple models with the same independent variables. The procedure calculates the matrix decomposition part of the calculations only once, regardless of the number of dependent variables in the model. These calculations are responsible for most of the computing time used by the TPSPLINE procedure. This feature is particularly useful when you need to generate a bootstrap confidence interval.
To construct a bootstrap confidence interval, perform the following tasks:
Fit the data by using PROC TPSPLINE and obtain estimates and .
Generate K bootstrap samples based on and .
Fit the K bootstrap samples with the TPSPLINE procedure to obtain estimates of and .
Compute and the values and .
The following statements illustrate this process:
proc tpspline data=melanoma plots(only)=fitplot(clm); model incidences = (year) /alpha = 0.1; output out=result pred uclm lclm; run;
The output from the initial PROC TPSPLINE analysis is displayed in Output 96.5.3. The data set result
contains the predicted values and confidence limits from the analysis.
Output 96.5.3: Output from PROC TPSPLINE for the MELANOMA Data
Comparison of Two Estimates 
with and without the D= Option 
Summary of Input Data Set  

Number of NonMissing Observations  37 
Number of Missing Observations  0 
Unique Smoothing Design Points  37 
Summary of Final Model  

Number of Regression Variables  0 
Number of Smoothing Variables  1 
Order of Derivative in the Penalty  2 
Dimension of Polynomial Space  2 
Summary Statistics of Final Estimation  

log10(n*Lambda)  0.0607 
Smoothing Penalty  0.5171 
Residual SS  1.2243 
Tr(IA)  22.5852 
Model DF  14.4148 
Standard Deviation  0.2328 
GCV  0.0888 
The following statements illustrate how you can obtain a bootstrap confidence interval for the Melanoma
data set. The following statements create the data set bootstrap
. The observations are created with information from the preceding PROC TPSPLINE execution; as displayed in Output 96.5.3, . The values of are stored in the data set result
in the variable P_incidence
.
data bootstrap; set result; array y{1070} y1y1070; do i=1 to 1070; y{i} = p_incidences + 0.232823*rannor(123456789); end; keep y1y1070 p_incidences year; run; ods listing close; proc tpspline data=bootstrap plots=none; ods output FitStatistics=FitResult; id p_incidences; model y1y1070 = (year); output out=result2; run; ods listing;
The DATA step generates 1,070 bootstrap samples based on the previous estimate from PROC TPSPLINE. For this data set, some of the bootstrap samples result in s (selected by the GCV function) that cause problematic behavior. Thus, an additional 70 bootstrap samples are generated.
The ODS listing destination is closed before PROC TPSPLINE is invoked. The PLOTS=NONE option suppresses all graphical output.
The model fits all the y1
…y1070
variables as dependent variables, and the models are fit for all bootstrap samples simultaneously. The output data set result2
contains the variables year
, y1
…y1070
, p_y1
…p_y1070
, and p_incidences
.
The ODS OUTPUT statement writes the FitStatistics
table to the data set FitResult
. The data set FitResult
contains the two variables Parameter
and Value
. The FitResult
data set is used in subsequent calculations for .
In the data set FitResult
, there are 63 estimates with a standard deviation of zero, suggesting that the estimates provide perfect fits of the data
and are caused by s that are approximately equal to zero. For small sample sizes, there is a positive probability that the chosen by the GCV function will be zero (Wang and Wahba, 1995).
In the following steps, these cases are removed from the bootstrap samples as “bad” samples: they represent failure of the GCV function.
The following SAS statements manipulate the data set FitResult
, retaining the standard deviations for all bootstrap samples and merging FitResult
with the data set result2
, which contains the estimates for bootstrap samples. In the final data set boot
, the statistics are calculated.
data FitResult; set FitResult; if Parameter="Standard Deviation"; keep Value; run; proc transpose data=FitResult out=sd prefix=sd; data result2; if _N_ = 1 then set sd; set result2; run; data boot; set result2; array y{1070} p_y1p_y1070; array sd{1070} sd1sd1070; do i=1 to 1070; if sd{i} > 0 then do; d = (y{i}  P_incidences)/sd{i}; obs = _N_; output; end; end; keep d obs P_incidences year; run;
The following SAS statements retain the first 1,000 bootstrap samples and calculate the values and with .
proc sort data=boot; by obs; run; data boot; set boot; by obs; retain n; if first.obs then n=1; else n=n+1; if n > 1000 then delete; run; proc sort data=boot; by obs d; run; data chi1 chi2 ; set boot; if (_N_ = (obs1)*1000+50) then output chi1; if (_N_ = (obs1)*1000+950) then output chi2; run; proc sort data=result; by year; run; proc sort data=chi1; by year; run; proc sort data=chi2; by year; run; data result; merge result chi1(rename=(d=chi05)) chi2(rename=(d=chi95)); keep year incidences P_incidences lower upper LCLM_incidences UCLM_incidences; lower = chi95*0.232823 + P_incidences; upper = chi05*0.232823 + P_incidences; label lower="Lower 90% CL (Bootstrap)" upper="Upper 90% CL (Bootstrap)" lclm_incidences="Lower 90% CL (Bayesian)" uclm_incidences="Upper 90% CL (Bayesian)"; run;
The data set result
contains the variables year
and incidences
, the PROC TPSPLINE estimate P_incidences
, and the 90% Bayesian and 90% bootstrap confidence intervals.
The following statements produce Output 96.5.4:
proc sgplot data=result; title "Ageadjusted Melanoma Incidence for 37 Years"; xaxis label="year"; yaxis label="Incidences"; band x=year lower=lclm_incidences upper=uclm_incidences/name="bayesian" legendlabel="90% Bayesian CI of Predicted incidences" fillattrs=(color=red); band x=year lower=lower upper=upper/name="bootstrap" legendlabel="90% Bootstrap CI of Predicted incidences" transparency=0.05; scatter x=year y=incidences/name="obs" legendlabel="incidences"; series x=year y=p_incidences/name="pred" legendlabel="predicted values of incidences" lineattrs=graphfit(thickness=1px); discretelegend "bayesian" "bootstrap" "obs" "pred"; run; ods graphics off;
Output 96.5.4 displays the plot of the variable incidences
, the predicted values, and the Bayesian and bootstrap confidence intervals.
The plot shows that the bootstrap confidence interval is similar to the Bayesian confidence interval. However, the Bayesian confidence interval is symmetric around the estimates, while the bootstrap confidence interval is not.
Output 96.5.4: Comparison of Bayesian and Bootstrap Confidence Interval for Data Set MELANOMA