# The HPFMM Procedure

### Mixture Modeling for Binomial Overdispersion: "Student," Pearson, Beer, and Yeast

The following example demonstrates how you can model a complicated, two-component binomial mixture distribution, either with maximum likelihood or with Bayesian methods, with a few simple PROC HPFMM statements.

William Sealy Gosset, a chemist at the Arthur Guinness Son and Company brewery in Dublin, joined the statistical laboratory of Karl Pearson in 1906–1907 to study statistics. At first Gosset—who published all but one paper under the pseudonym "Student" because his employer forbade publications by employees after a co-worker had disclosed trade secrets—worked on the Poisson limit to the binomial distribution, using haemacytometer yeast cell counts. Gosset’s interest in studying small-sample (and limit) problems was motivated by the small sample sizes he typically saw in his work at the brewery.

Subsequently, Gosset’s yeast count data have been examined and revisited by many authors. In 1915, Karl Pearson undertook his own examination and realized that the variability in "Student’s" data exceeded that consistent with a Poisson distribution. Pearson (1915) bemoans the fact that if this were so, "it is certainly most unfortunate that such material should have been selected to illustrate Poisson’s limit to the binomial."

Using a count of Gosset’s yeast cell counts on the 400 squares of a haemacytometer (Table 6.1), Pearson argues that a mixture process would explain the heterogeneity (beyond the Poisson).

Table 6.1: "Student’s" Yeast Cell Counts

Number of Cells

0

1

2

3

4

5

Frequency

213

128

37

18

3

1

Pearson fits various models to these data, chief among them a mixture of two binomial series where is real-valued and thus the binomial series expands to Pearson’s fitted model has , , (corresponding to a mixing proportion of ), and estimated success probabilities in the binomial components of 0.1017 and 0.4514, respectively. The success probabilities indicate that although the data have about a 90% chance of coming from a distribution with small success probability of about 0.1, there is a 10% chance of coming from a distribution with a much larger success probability of about 0.45.

If is an integer, the binomial series is the cumulative mass function of a binomial random variable. The value of suggests that a suitable model for these data could also be constructed as a two-component mixture of binomial random variables as follows: The binomial sample size n=5 is suggested by Pearson’s estimate of and the fact that the largest cell count in Table 6.1 is 5.

The following DATA step creates a SAS data set from the data in Table 6.1.

data yeast;
input count f;
n = 5;
datalines;
0     213
1     128
2      37
3      18
4       3
5       1
;


The two-component binomial model is fit with the HPFMM procedure with the following statements:

proc hpfmm data=yeast;
model count/n =  / k=2;
freq f;
run;


Because the events/trials syntax is used in the MODEL statement, PROC HPFMM defaults to the binomial distribution. The K= 2 option specifies that the number of components is fixed and known to be two. The FREQ statement indicates that the data are grouped; for example, the first observation represents 213 squares on the haemacytometer where no yeast cells were found.

The "Model Information" and "Number of Observations" tables in Figure 6.1 convey that the fitted model is a two-component homogeneous binomial mixture with a logit link function. The mixture is homogeneous because there are no model effects in the MODEL statement and because both component distributions belong to the same distributional family. By default, PROC HPFMM estimates the model parameters by maximum likelihood.

Although only six observations are read from the data set, the data represent 400 observations (squares on the haemacytometer). Since a constant binomial sample size of 5 is assumed, the data represent 273 successes (finding a yeast cell) out of 2,000 Bernoulli trials.

Figure 6.1: Model Information for Yeast Cell Model

The HPFMM Procedure

Model Information
Data Set YEAST
Response Variable (Events) count
Response Variable (Trials) n
Frequency Variable f
Type of Model Homogeneous Mixture
Distribution Binomial
Components 2
Link Function Logit
Estimation Method Maximum Likelihood

 Number of Observations Read 6 6 400 400 273 2000

The estimated intercepts (on the logit scale) for the two binomial means are –2.2316 and –0.2974, respectively. These values correspond to binomial success probabilities of 0.09695 and 0.4262, respectively (Figure 6.2). The two components mix with probabilities 0.8799 and . These values are generally close to the values found by Pearson (1915) using infinite binomial series instead of binomial mass functions.

Figure 6.2: Maximum Likelihood Estimates

Parameter Estimates for Binomial Model
Component Parameter Estimate Standard
Error
z Value Pr > |z| Inverse Linked
Estimate
1 Intercept -2.2316 0.1522 -14.66 <.0001 0.09695
2 Intercept -0.2974 0.3655 -0.81 0.4158 0.4262

Parameter Estimates for Mixing Probabilities
Component Mixing Probability Linked Scale
Logit(Prob) Standard
Error
z Value Pr > |z|
1 0.8799 1.9913 0.5725 3.48 0.0005
2 0.1201 -1.9913

To obtain fitted values and other observationwise statistics under the stipulated two-component model, you can add the OUTPUT statement to the previous PROC HPFMM run. The following statements request componentwise predicted values and the posterior probabilities:

proc hpfmm data=yeast;
model count/n =  / k=2;
freq f;
id f n;
output out=hpfmmout pred(components) posterior;
run;
data hpfmmout;
set hpfmmout;
PredCount_1 = post_1 * f;
PredCount_2 = post_2 * f;
run;
proc print data=hpfmmout;
run;


The DATA step following the PROC HPFMM step computes the predicted cell counts in each component (Figure 6.3). Note that the The predicted means in the components, 0.48476 and 2.13099, are close to the values determined by Pearson (0.4983 and 2.2118), as are the predicted cell counts.

Figure 6.3: Predicted Cell Counts

Obs f n Pred_1 Pred_2 Post_1 Post_2 PredCount_1 PredCount_2
1 213 5 0.096951 0.42620 0.98606 0.01394 210.030 2.9698
2 128 5 0.096951 0.42620 0.91089 0.08911 116.594 11.4058
3 37 5 0.096951 0.42620 0.59638 0.40362 22.066 14.9341
4 18 5 0.096951 0.42620 0.17598 0.82402 3.168 14.8323
5 3 5 0.096951 0.42620 0.02994 0.97006 0.090 2.9102
6 1 5 0.096951 0.42620 0.00444 0.99556 0.004 0.9956

Gosset, who was interested in small-sample statistical problems, investigated the use of prior knowledge in mathematical-statistical analysis—for example, deriving the sampling distribution of the correlation coefficient after having assumed a uniform prior distribution for the coefficient in the population (Aldrich, 1997). Pearson also was not opposed to using prior information, especially uniform priors that reflect "equal distribution of ignorance." Fisher, on the other hand, would not have any of it: the best estimator in his opinion is obtained by a criterion that is absolutely independent of prior assumptions about probabilities of particular values. He objected to the insinuation that his derivations in the work on the correlation were deduced from Bayes theorem (Fisher, 1921).

The preceding analysis of the yeast cell count data uses maximum likelihood methods that are free of prior assumptions. The following analysis takes instead a Bayesian approach, assuming a beta prior distribution for the binomial success probabilities and a uniform prior distribution for the mixing probabilities. The changes from the previous run of PROC HPFMM are the addition of the ODS GRAPHICS, PERFORMANCE , and BAYES statements and the SEED= 12345 option.

ods graphics on;
proc hpfmm data=yeast seed=12345;
model count/n = / k=2;
freq f;
performance nthreads=2;
bayes;
run;
ods graphics off;


When ODS Graphics is enabled, PROC HPFMM produces diagnostic trace plots for the posterior samples. Bayesian analyses are sensitive to the random number seed and thread count; the SEED= and NTHREADS= options in the PERFORMANCE statement ensure consistent results for the purposes of this example. The SEED= 12345 option in the PROC HPFMM statement determines the random number seed for the random number generator that the analysis used. The NTHREADS=2 option in the PERFORMANCE statement sets the number of threads to be used by the procedure to two. The BAYES statement requests a Bayesian analysis.

The "Bayes Information" table in Figure 6.4 provides basic information about the Markov chain Monte Carlo sampler. Because the model is a homogeneous mixture, the HPFMM procedure applies an efficient conjugate sampling algorithm with a posterior sample size of 10,000 samples after a burn-in size of 2,000 samples. The "Prior Distributions" table displays the prior distribution for each parameter along with its mean and variance and the initial value in the chain. Notice that in this situation all three prior distributions reduce to a uniform distribution on .

Figure 6.4: Basic Information about MCMC Sampler

The HPFMM Procedure

Bayes Information
Sampling Algorithm Conjugate
Data Augmentation Latent Variable
Initial Values of Chain Data Based
Burn-In Size 2000
MC Sample Size 10000
MC Thinning 1
Parameters in Sampling 3
Mean Function Parameters 2
Scale Parameters 0
Mixing Prob Parameters 1

Prior Distributions
Component Parameter Distribution Mean Variance Initial
Value
1 Success Probability Beta(1, 1) 0.5000 0.08333 0.1365
2 Success Probability Beta(1, 1) 0.5000 0.08333 0.1365
1 Probability Dirichlet(1, 1) 0.5000 0.08333 0.6180

The HPFMM procedure produces a log note for this model, indicating that the sampled quantities are not the linear predictors on the logit scale, but are the actual population parameters (on the data scale):

NOTE: Bayesian results for this model (no regressor variables,
non-identity link) are displayed on the data scale, not the
linked scale. You can obtain results on the linked (=linear)
scale by requesting a Metropolis-Hastings sampling algorithm.


The trace panel for the success probability in the first binomial component is shown in Figure 6.5. Note that the first component in this Bayesian analysis corresponds to the second component in the MLE analysis. The graphics in this panel can be used to diagnose the convergence of the Markov chain. If the chain has not converged, inferences cannot be made based on quantities derived from the chain. You generally look for the following:

• a smooth unimodal distribution of the posterior estimates in the density plot displayed on the lower right

• good mixing of the posterior samples in the trace plot at the top of the panel (good mixing is indicated when the trace traverses the support of the distribution and appears to have reached a stationary distribution)

Figure 6.5: Trace Panel for Success Probability in First Component The autocorrelation plot in Figure 6.5 shows fairly high and sustained autocorrelation among the posterior estimates. While this is generally not a problem, you can affect the degree of autocorrelation among the posterior estimates by running a longer chain and thinning the posterior estimates; see the NMC= and THIN= options in the BAYES statement.

Both the trace plot and the density plot in Figure 6.5 are indications of successful convergence.

Figure 6.6 reports selected results that summarize the 10,000 posterior samples. The arithmetic means of the success probabilities in the two components are 0.0917 and 0.3974, respectively. The posterior mean of the mixing probability is 0.8312. These values are similar to the maximum likelihood parameter estimates in Figure 6.2 (after swapping components).

Figure 6.6: Summaries for Posterior Estimates

Posterior Summaries
Component Parameter N Mean Standard
Deviation
Percentiles
25 50 75
1 Success Probability 10000 0.0917 0.0168 0.0830 0.0938 0.1027
2 Success Probability 10000 0.3974 0.0871 0.3379 0.3957 0.4556
1 Probability 10000 0.8312 0.1045 0.7986 0.8578 0.8984

Posterior Intervals
Component Parameter Alpha Equal-Tail Interval HPD Interval
1 Success Probability 0.050 0.0530 0.1187 0.0585 0.1212
2 Success Probability 0.050 0.2272 0.5722 0.2343 0.5780
1 Probability 0.050 0.5464 0.9454 0.6325 0.9642

Note that the standard errors in Figure 6.2 are not comparable to those in Figure 6.6, since the standard errors for the MLEs are expressed on the logit scale and the Bayes estimates are expressed on the data scale. You can add the METROPOLIS option in the BAYES statement to sample the quantities on the logit scale.

The "Posterior Intervals" table in Figure 6.6 displays 95% credible intervals (equal-tail intervals and intervals of highest posterior density). It can be concluded that the component with the higher success probability contributes less than 40% to the process.