Running the %TPLOT macro creates a window displaying a graphical representation of the marker test results.
Figure 12.1 shows an example of the TPLOT results window.
This plot contains a grid of points with symbols that represent the -values for various marker tests. Colors and shapes of the data points are used to symbolize -value ranges. The Show Info About Points button on the toolbar enables the -values to be displayed. If you hold down the left mouse button while positioning the mouse pointer on any point in the plot, the pop-up window appears for off-diagonal points, displaying the two markers being tested for linkage disequilibrium and the -value of the test; the window displays the marker and its -values for the HWE test and marker-trait association test for points on the diagonal, as shown in Figure 12.1.
The -values from the linkage disequilibrium tests between all pairs of markers (or all markers within a certain range of each other) are represented by the color of the squares on the off-diagonal of the plot. For the points on the diagonal, the results from the Hardy-Weinberg equilibrium test are displayed instead of the linkage disequilibrium tests since the same marker locus is on the horizontal and vertical axes.
The three ranges of -values that correspond to different colored symbols in the plot are as follows:
[0, 0.01]
(0.01, 0.05]
(0.05, 1]
The disequilibrium test -values that are plotted can be provided by the output data set from PROC ALLELE, or by a user-created data set meeting the requirements described in the Syntax: TPLOT Macro section.
Points on the diagonal also display -values from marker-trait association tests, using the shape of the symbol to correspond to two categories of -values, significant and not significant. The significance level is set to 0.05 by default, but can be modified using the ALPHA= option in the %TPLOT macro. Thus, for a significance level of , the following shapes represent the following ranges:
[0, ]
(, 1]
Note that the square shape of the off-diagonal points does not represent a marker-trait association -value since there are two different marker loci represented on the horizontal and vertical axes. These -values can be provided by the output data set of PROC CASECONTROL, PROC FAMILY, or PROC PSMOOTH. Alternatively, a user-created data set that meets the conditions described in the Syntax: TPLOT Macro section can be used.